Bio3400 Chapter 6 Genetic Analysis and Mapping in Bacteria and Bacteriophages
  1. Bacterial growth can be divided into three phases:      phase,      phase (              growth), and             phase.

  2. Cells grown in liquid medium can be quantified by the serial            technique.

  3. Bacteria can undergo              , in which genetic information from one bacterium is transferred to another, resulting in                in the latter.

  4. F+ cells contain a            (F) factor that has genes for sex        formation and allows the host to donate DNA during              . Recipient cells are usually converted to F+.

  5. An      (high-frequency recombination) strain has an             F factor, and can donate DNA in an           fashion to an F- cell.

  6. Sometimes, an F factor is          from the chromosome of an Hfr cell, producing an     cell. Transfer of an F' to an F- cell results in a             .

  7.           such as    Factors contain one or more genes and replicate independently of the bacterial chromosome.    plasmids confer antibiotic resistance.

  8. Bacteria can also undergo genetic recombination by                 , in which pieces of extracellular DNA are taken up by a cell and             into the chromosome.

  9.                 (phages) such as T4 are viruses that infect a host bacterium and use the host enzymes to reproduce.

  10. The number of phages produced following the infection of bacteria can be determined by the         assay.

  11. Sometimes a            phage integrates its DNA into the bacterial chromosome in a process called           , and the viral DNA (           ) is replicated with the host DNA.

  12. Bacteriophages can mediate genetic                in their bacterial hosts in a process called               .

    • In              transduction,            DNA instead of        DNA is packaged in a phage particle and transferred to a recipient host.

  13.             mapping in bacteriophages can be done by examining phage mutations that affect plaque             .